PRAZO: 14 Abril 2025
Calls da Indústria
Calls Abertas
PRAZO: 28 Abril 2025
Novel Therapeutic Approaches for Genetic Skeletal Diseases and Targeted Delivery to Bone
PRAZO: 28 Abril 2025
Molecular Strategies for Targeted Drug Delivery to the Joints
![BACKGROUND The congenital malformations, arthritis, osteoporosis, traumatic accidents, spinal deformities, among others are the basis for need bone and cartilage replacements. The chronic pain, inflammation or discomfort of these diseases translates into a significant impact on patients’ and hospital treatments costs. There is yet no solution that meets the structural and functional requirements of definitive implants. In the case of trauma, for example, treatment often consists of bone joining or even implants in a nonpermanent situation, that is, requiring more than one surgical intervention which causes pain and discomfort to the patient besides the costs involved. Therefore, the development of low cost, durable, compatible and functional solutions is needed. Namely bone substitutes that can be perfectly integrated, morphologically and anatomically biocompatible, with complex structure and a material that mimics native bone and encourages cell migration, proliferation and differentiation. Ideally a bone substitute has original three-dimensional anatomical shape the trabecular zone is dense and has Young’s modulus in the range of 0.01-0.5 GPa and compressive strength values of 4-12 MPa while the cortical region is porous with compressive strength values in the range 130-225 MPa and modulus Young from 3-30GPa. The development of bone implants represents an area of medicine attracting growing interest in recent years. Most used implants are made of noble metals such as titanium. This material, although with good biocompatibility, strength and durability characteristics, faces often body rejection problems. To avoid that, the metal is coated with a biocompatible ceramic material (zirconia, hydroxyapatite, etc.). Despite their good performance, titanium implants have a much higher Young’s modulus than bone, which leads to localized stress phenomena, increase of osteoblast activity and may lead to periprosthetic osteolysis and consequent implant failure. The 3D printing techniques provided opportunities for the development of customized pieces. Ceramics, plastics and metals are all possible to printing in three dimensions by additive or subtractive techniques. In particular subtractive techniques are already used to perform ceramic teeth for dentistry. A piece of dense ceramic material is mechanically dug until the desired form is obtained. However, the dense ceramic implant behaves most likely the metal implants as it doesn’t allow the body fluids to flow through it. Compared to other techniques, the great advantage of pastes extrusion technique is the facility to mimic the graded porosity of implants while a specific design according to patient needs, reaching the mechanical properties close to those of the native bone, as well as their adequate, porosity, permeability to cells, biocompatibility and durability. The goal of the NOVA researchers is to develop low cost production ceramic implants, permanent, conformal and compatible with the specific needs of patients, mimicking resistance and bioactivity. This approach was achieved in preliminary results focused in 3D printed pieces of ceramics powders, achieving mechanical properties in the range of cortical bone, the most demanding in terms of mechanical strength. Indeed, the cortical mechanical resistance values were achieved just printing pieces layer-by-layer with pastes made with inorganic and organic compounds. The required biocompatibility and osteointegration was also tested in-vivo. TECHNOLOGY OVERVIEW The obtained background data show a real possibility to implement this technology in the veterinary area special to horses, dogs and cats in a first phase. This will open then possibilities and verification to further human applications. From left to right: Sample draw; printed samples after sintering for compression test; geometry and dimensions to tension tests; samples geometry for flexure tests. The mechanical strength obtained from axial compression, the tension and the flexure tests are summarized in table 1, where the obtained values are compared to the trabecular and cortical bone values obtained from literature. Comparison of mechanical teste with bone (values from literature).[1][2] Density and porosity compared to bone. The in-vitro tests were made with SAOS 2 cellules tinted for alkaline phosphatase with naphthol AS-MX phosphate and fast red violet and images of cell proliferation obtained for day 5 (left image) and 11 day (right image) of culture are shown in Figure 2. Figure 2: Image of SAOS 2 cells on the top a PIC sample for day 5 (left) and day 11 (right). The graph shows the evolution of cells growth along 9 days. The in-vivo tests were performed in rabbit being implanted two different samples S1 and S2 and the radiography images obtained after 2, 4 and 12 weeks are shown in Figure 3. Figure 3: Image of the implant (left). The graph represents the % of the bone-to-implant contact (BIC) evolution for two different composition samples. There was no adverse response, either local or systemic. No clinical symptoms of exaggerated inflammation, edema or infection were identified. No extensive bone resorption of the surrounding bone tissue is identified STAGE OF DEVELOPMENT The Technology Readiness Level (TRL) is 4. BENEFITS The main advantage of this technology is to allow the printing of a customized bone according to the needs of replacement. It enables the production of bone with gradient of density, in the exterior, properties similar to cortical bone, and, in the interior, density and properties of trabecular bone. Adding to that, the good osteointegration of the material, will allow a giant step towards bone’s mimicking especially for implants in animals, as a first step. Avoiding the need for a second operation for removing metallic parts, or due to rejection problems. APPLICATIONS The potential market is the implants in animals, horses, dogs and cats. There is a huge market for pets, which will allow also to consolidate the technology before it can be implemented in humans. OPPORTUNITY The NOVA team is looking for a co-development partner or a licensee for the implementation of this technology. IP STATUS Know-how based SEEKING Development partner Licensing](https://novainnovation.unl.pt/wp-content/uploads/2021/02/tech_image_2.7ccfbcf1.jpg)
PRAZO: 05 Maio 2025
Seeking Novel Solutions to Treat Rare Genetic Skeletal Muscle Diseases
PRAZO: 19 Maio 2025
Novel Technologies for Drug Delivery to Treat Genetic Cardiomyopathy
PRAZO: 19 Maio 2025
Therapeutic Discovery for Laminopathies
Calls Encerradas
CALL ENCERRADA: 11 Abril 2022
Processes of Green Carbon Removal from Refractories for Recycling
CALL ENCERRADA: 11 Abril 2022
Seeking Transformative Solutions to Treat Rare Skeletal Diseases
CALL ENCERRADA: 11 Abril 2022
Research Opportunities for C-type Natriuretic Peptide
CALL ENCERRADA: 18 Abril 2022
Generation of iPSC-derived T Cells for Therapeutic Use
CALL ENCERRADA: 28 Março 2022
Seeking Bioderived Thermoplastic-like Resin Suitable for Vacuum Infusion or Resin Transfer Moulding
CALL ENCERRADA: 28 Março 2022
Improving the Fermentation of Filamentous Fungi
CALL ENCERRADA: 21 Março 2022
Therapeutics and Drug Candidates in Immuno-Oncology
CALL ENCERRADA: 21 Março 2022
Methods of CO2 Conversion to Chemical Products by Catalytic Routes
CALL ENCERRADA: 21 Fevereiro 2022
Seeking Translational Early Discovery Research in Haematology
CALL ENCERRADA: 07 Março 2022
Novel Therapeutics Targeting Disease/Tissue-specific Macrophage Biology with a Focus on Regeneration and Clearance
CALL ENCERRADA: 24 Janeiro 2022
Advanced Materials for the Generation of Bio-Based Products via Immobilised Non-Mammalian Organisms
CALL ENCERRADA: 17 Janeiro 2022
Seeking Differentiated RNA Technologies for Vaccines
CALL ENCERRADA: 17 Janeiro 2022
Plant Based Protein for Human Health, Industrial Applications and Sustainable Agriculture
CALL ENCERRADA: 10 Janeiro 2022
Insights into Polycystic Kidney Disease Pathogenesis
CALL ENCERRADA: 10 Janeiro 2022
Therapeutic Small Molecule Regulators of RNA Transcripts
CALL ENCERRADA: 15 Novembro 2021
Seeking Genetically-Based Early Discovery Research in Nephrology
CALL ENCERRADA: 01 Novembro 2021
Seeking Monogenic Targets for RNA Interference-Based Therapy
CALL ENCERRADA: 08 Novembro 2021
Therapeutic Agents for Kidney Repair
CALL ENCERRADA: 25 Outubro 2021
New Therapeutic Strategies Targeting Myeloid Malignancies
CALL ENCERRADA: 18 Outubro 2021
Mechanism of Exosome Uptake from the Gastrointestinal Tract
CALL ENCERRADA: 04 Outubro 2021
Novel Complement Pathway Targets for the Treatment of Peripheral and Nervous System Inflammatory Disorders
CALL ENCERRADA: 27 Setembro 2021
State-of-the-Art Polymer Coating Technologies for Flexible Barrier Packaging
CALL ENCERRADA: 11 Agosto 2021
Autoimmune & Inflammatory Focused Drug Discovery and Early Pre-Clinical Development
CALL ENCERRADA: 23 Agosto 2021
Seeking Redevelopment Opportunities for the Treatment of Rare or Orphan Diseases
CALL ENCERRADA: 29 Julho 2021
Innovative DMPK and Safety Research for Therapeutics and Drug Delivery Systems
CALL ENCERRADA: 29 Julho 2021
Innovations in Platform Technologies: New Modalities and Drug Delivery Systems
CALL ENCERRADA: 29 Julho 2021
Novel Targets and Treatments in Oncology and Immunology
CALL ENCERRADA: 29 Julho 2021
Innovative Therapeutic Targets, Drugs, Disease Mechanisms and Technologies in Neuroscience
CALL ENCERRADA: 19 Julho 2021
In-Silico, Predictive Formulation Development Tools and Computational Methods
CALL ENCERRADA: 19 Julho 2021
Controlled Release of Orally Delivered Dosage Forms
CALL ENCERRADA: 10 Maio 2021
Innovations to Treat and Diagnose Lymphatics-Based Diseases
CALL ENCERRADA: 26 Abril 2021
Improving the Precision of Immunotherapies Through Cell Targeting
CALL ENCERRADA: 26 Abril 2021
New Therapeutic Strategies Promoting Resolution of Inflammation
CALL ENCERRADA: 26 Abril 2021
Novel Approaches Defining the Role of Gut Barrier Dysfunction in Driving Systemic Inflammation and Disease
CALL ENCERRADA: 19 Abril 2021
Seeking Novel Gene Regulatory Elements with Data to Confirm Efficacy in Plants
CALL ENCERRADA: 19 Abril 2021
High Throughput Plant Regulatory Element Evaluation Capability
CALL ENCERRADA: 05 Abril 2021
Seeking Phenotypic Cellular Assays Relating to Gastroenterology
CALL ENCERRADA: 05 Abril 2021
Seeking Phenotypic Cellular Assays Relating to Neuroscience
CALL ENCERRADA: 05 Abril 2021
Seeking Phenotypic Cellular Assays Relating to Oncology
CALL ENCERRADA: 05 Abril 2021
Seeking Phenotypic Cellular Assays Relating to Rare Disease
CALL ENCERRADA: 09 Agosto 2021
Innovative Therapies for the Treatment of SkinDisorders
CALL ENCERRADA: 09 Agosto 2021