BioLab – Biological and Chemical Analysis Facility [NI-SERV-2020-42]
The BioLab gathers in a single unit state-of-the-art biophysical and biological technologies, devoted to the comprehension of cellular and molecular mechanisms
Service/Expertise Overview
Circular Dichroism – Applied Photophysics Chirascan qCD spectrometer
Modern and powerful device that allows fast and sensitive analysis. Additionally, the SX20 Stopped Flow accessory allows CD and absorbance stopped-flow measurements of high sensitivity.
Differential Scanning Calorimetry – TA Nano DSC
Powerful thermal scanning instrument that utilizes a 300 µL capillary cell design and solid-state thermoelectric temperature control to provide unmatched performance.
Flow Cytometry – Attune Acoustic Focusing Cytometer
2-laser (red and blue), 6-colours system that uses ultrasonic waves in combination with hydrodynamic forces allowing the collection of more photons for high-precision analysis at superior volumetric sample throughput.
MicroScale Thermophoresis – NanoTemper technologies Monolith NT.115 (BLUE/RED)
Measures biomolecular interactions providing a broad application range to detect interactions between any type of biomolecules and for pM to mM binding affinities.
Multi Parametric Surface Plasmon Resonance – Bionavis SPR Navi 200
Improved and versatile system, that uses an increased angular scan range to produce a complete SPR curve, enabling new insights into interactions. This increased angular range also allows the characterization of the interactions of surfaces with analytes, with molecular weights ranging from a few Da to several kDa, in the gas or liquid phases.
Competitive advantages
The BioLab has a unique set of state-of-the-art technologies available and is devoted to the comprehension of cellular and molecular mechanisms, this facility provides services to the industry and academia with competitive prices and specialized support.
The microscale thermophoresis equipment is the only one available in Portugal and measures a broad range of binding affinities in-solution, using very little sample in close-to-native conditions.
The MP-SPR device is the only MP-SPR in the country equipped with a gas flow cell, unlike traditional SPR, our MP-SPR can work with gas and vapour samples. It can be used to fine-tune sensitive coatings for specific gas components.
Applications
- Circular Dichroism:
- Proteins;
- Secondary/tertiary structure analysis;
- Detection of conformational changes;
- Study of binding interactions.
- Nucleic acids;
- Detection of conformational changes;
- Organic compounds;
- Differential Scanning Calorimetry:
- Protein stability and folding;
- Binding interactions;
- Flow Cytometry;
- Apoptosis;
- Immunophenotyping;
- Cell cycle analysis;
- Microbiological applications;
- MicroScale Thermophoresis;
- KD determination;
- Interactions in complex liquids;
- Competition assays;
- MP-SPR;
- Molecular interactions;
- Layer properties.
Further details
Research centre
UCIBIO
